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  Authority of Polish Society of Allergology

vol 11. no 1. March 2006  

 Original articles
Serum concentration of sCD30, Annexin V auto-antibodies and Bcl-2 level in lymphocytes lysates obtained from patients with inhalant allergy
Sławomir Żegleń, Barbara Rogala

Introduction. The importance of apoptosis regulating factors in the pathophysiology of atopic disorders is still disputable. CD30 as an apoptosis regulating receptor is suspected to be involved in the immunocompetent cell survival rate that is increased during allergic inflammation.
Aim of the study. The aim of presented work was to evaluate the sCD30 molecule concentration in sera obtained from atopic patients and to analyse the relationship between the concentrations of sCD30 and anti-Annexin V antibodies and Bcl-2 oncoprotein.
Material and methods. 52 grass allergen-sensitive patients suffering from seasonal rhinitis and/or seasonal asthma, and 26 subjects with mild allergic asthma sensitised to house dust mite antigens were included into the trial. Control group consisted of 24 subjects without any signs of atopy. Immunoenzyme assay for sCD30, Bcl-2 and Anti-annexin V antibodies detection was used (DAKO CD30, ELISA, Bcl-2 Bender MedSystems, Bender MedSystems - anti-Annexin V ELISA). Fluoroimmunoenzyme method was employed for TIgE estimation.
Results. The sCD30 concentration was significantly increased in patients suffering from allergic rhinitis and/or seasonal asthma compared to healthy control (median, P - 17.0 U/mL vs K - 8.05 U/mL, AS1 - 21.5 U/mL vs. K - 8.05 U/mL, p<0.05). Serum sCD30 concentrations in the asthmatic patients sensitised to house dust mite were not different from those noted in the control (median, AS2 - 10.3 U/mL vs. K - 8.05 U/mL, p>0.05). Bcl-2 molecule concentration in lymphocyte lysates isolated from the atopic subjects was comparable with that determined in the control subjects (median, AT1 350 - U/mL vs. K - 277.5 U/mL; p>0.05). The prevalence of anti-Annexin V antibodies was comparable in control and atopic patients ( 47% vs. 54%, respectively). There was no relationship between the concentrations of the studied molecules.
Conclusions. sCD30 could be involved in pathophysiology of some sensitisations. There is no relationship between apoptosis-regulating molecules, such as anti-annexin V antibodies and Bcl-2, and sCD30 concentration.

keywords: sCD30, Bcl-2, przeciwciała anti-Annexin V, alergia, apoptoza, sCD30, Bcl-2, anti-Annexin V antibodies, allergy, apoptosis

pages: from 35 to 41

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